In this research, IL-22 being expressed in 4T1 cancer cell has been accepted. When looking at the data, unstained cells, whether its red or green dye, expressed less than 1% of fluorescence, concluding that those cells do not naturally glow in that certain wavelengths of light. In contrast, the red-stained cells expressed 62.51% fluorescence, indicating that IL-22 is present in 4T1s.
This correlation between MDSCS and 4T1 cells was expected, as previous research has proven the correlation between MDSCs and cancer. In relation to GFP expression, the results are not as exquisite. For unstained cells, .24% of the cells displayed any correlation between GFP on 4T1. With the stain, 5.05% expressed GFP on the cell line. As there is a 4.81% increase, it is not sufficient enough to conclude that the GFP gene is very sufficient and trustworthy for future data. The lower increase in percentage means that the plasmid transfection of IL-22 knockout was not unquestionably positive. Because of the low percentage, the date was not usable for future research or to test the hypothesis any farther. To accomplish a true gene knockout, it is necessary for the transfected cells to grow to confluence, which was not able to be done in the time of this experiment. Concluding, the IL-22 knockout within this research was not accomplished. The data depicted from this research was not able to proceed enough to support or deny the hypothesis stated.
For future experiments and research, a successful knockout of IL-22 would be discovered with more time being a factor in forming the knockout. It is predicted that a significant amount of fluorescence to clarify a successful knockout would be over 50%. To verify, one should put a culture of purely modified cells through the cell sorter, separate out green or red cells. These cells could then be taken out and grown into cell lines. these cells could soon enough be back inserted into the balb/c mice to test on a human-like specimen. The cultured cells would need time to grow, exposing all that it possibly can. The tdTomato dye would be used to track the progress, allowing time to successfully define a gene knockout.
Immunotherapy is the most relevant treatment of cancer at the moment in the medical field. One of the major setbacks in designing an antibody against cancerous cells is MDSCs suppressing the actions of the immune system. It is possible that a drug could be designed that suppresses the workings of MDSCs, making it more realistic and plausible. On the other hand, CRISPR is not sufficient enough for the testing of cancer cell solutions. Through the relationship of IL-22 expression and MDSCs, past data is still very relevant in the work. unfortunately, the time provided for this experiment was not sufficient, and the research could not be completed to its full extent.
This correlation between MDSCS and 4T1 cells was expected, as previous research has proven the correlation between MDSCs and cancer. In relation to GFP expression, the results are not as exquisite. For unstained cells, .24% of the cells displayed any correlation between GFP on 4T1. With the stain, 5.05% expressed GFP on the cell line. As there is a 4.81% increase, it is not sufficient enough to conclude that the GFP gene is very sufficient and trustworthy for future data. The lower increase in percentage means that the plasmid transfection of IL-22 knockout was not unquestionably positive. Because of the low percentage, the date was not usable for future research or to test the hypothesis any farther. To accomplish a true gene knockout, it is necessary for the transfected cells to grow to confluence, which was not able to be done in the time of this experiment. Concluding, the IL-22 knockout within this research was not accomplished. The data depicted from this research was not able to proceed enough to support or deny the hypothesis stated.
For future experiments and research, a successful knockout of IL-22 would be discovered with more time being a factor in forming the knockout. It is predicted that a significant amount of fluorescence to clarify a successful knockout would be over 50%. To verify, one should put a culture of purely modified cells through the cell sorter, separate out green or red cells. These cells could then be taken out and grown into cell lines. these cells could soon enough be back inserted into the balb/c mice to test on a human-like specimen. The cultured cells would need time to grow, exposing all that it possibly can. The tdTomato dye would be used to track the progress, allowing time to successfully define a gene knockout.
Immunotherapy is the most relevant treatment of cancer at the moment in the medical field. One of the major setbacks in designing an antibody against cancerous cells is MDSCs suppressing the actions of the immune system. It is possible that a drug could be designed that suppresses the workings of MDSCs, making it more realistic and plausible. On the other hand, CRISPR is not sufficient enough for the testing of cancer cell solutions. Through the relationship of IL-22 expression and MDSCs, past data is still very relevant in the work. unfortunately, the time provided for this experiment was not sufficient, and the research could not be completed to its full extent.